Job title: Post-Doctoral Fellow

Reporting to: Principal Investigator – Dr. Nigel Stevenson

Location: Immunology Lab, RCSI Bahrain

Duration: 1 year

Objective:

A post-doctoral researcher is required from February/March 2021 in the Immunology Group, RCSI-Bahrain, based in the new, state of the art, RCSI/KHUH Clinical and Biomolecular Research Laboratory based in King Hamad University Hospital.

The successful candidate will join a program of research investigating the regulation of innate immune signalling, with a specific focus on intracellular signalling cascades, such as the JAK/STAT pathway, in both macrophages and neutrophils.

The project will use in vitro expression of genes and human blood samples. A major component of this translational project will be the design and optimisation of novel therapeutics to enhance the immune response.

General duties include:

• Ensuring that all Research and RCSI Bahrain policies and procedures are adhered to
• Complying with statutory legislation and RCSI Bahrain rules and requirements in furtherance of your own and general staff welfare and safety

·Undergoing programmes of training and development as may be required

·Representing the best interest of RCSI Bahrain and the Research Department at all times

·Performing such other duties as may be required from time to time

Required Qualifications:

Ph.D in Immunology, Biochemistry, Molecular Biology or a related biomedical subject.

Required Experience in:

·Human blood processing (PBMCs, neutrophils and serum isolation)

·Tissues culture of cell lines

·Real-Time qRT-PCR

·Flow cytometry (intracellular and extracellular analysis)

·Immunoblotting

·Molecular cloning of genes into DNA constructs and sequencing analysis

·Over-expression and knock-down of genes by tansfection

·Bioinformatic Analysis.

·Statistical Analysis

·Manuscript preparation/writing

Desirable experience in:

· CRISPR gene knockdown

· Nanostring analysis

· ELISA Genie multiplex analysis